Histological staining properties of Khaya senegalensis wood dust extracts: a preliminary study

*Nkiruka C. Azubuike, Ikenna T. Ikele, Anulika O. Onyemelukwe, Uzoamaka C. Maduakor, Okechukwu S. Onwukwe, Chioma L. Maduakor-Oparah, Ikechukwu J. Chukwu, Rita O. Okoye

Abstract

Crude aqueous and methanol extracts of Khaya senegalensis wood dust were employed as histological stains for the demonstration of general tissue architecture. Preliminary phytochemical screening of the extracts was conducted. Mixtures of the extracts of K. senegalensis in acidic, alkaline and neutral solutions were used to stain tissue sections. Preliminary phytochemical screening revealed that K. senegalensis extracts contains flavonoids, saponins, tannins, glycosides and reducing sugars. Optimum staining time was 15 minutes for both extracts and they stained the tissues in different shades of red and brown in the various media used. The best and worst stain uptake of tissues was observed with both extracts in alkaline and neutral media respectively. Mordant preparation of the extracts produced no significant staining difference from the non-mordant preparations. Staining solutions of the extracts have better affinity for the cellular cytoplasm and hence gave a good contrast when compared with Hematoxylin and Eosin control sections. In conclusion, Khaya senegalensis wood extract is a promising histological stain for the demonstration of general tissue architecture.

Keywords: Khaya senegalensis, wood dust, stain, histology, mordant

 


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